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Home Genome Resources

Gene and EST Database

Assembly V4.0: The A. mexicanum transcriptome has been deep sequenced (15,000,000 Roche 454 short sequence reads and 50K Sanger reads) and assembled. The resulting assembly V4.0 can now be BLAST searched here. Approximately 89.9% of the 14 million high quality sequences were assembled into contigs with a total length of 542 Mb.

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Genetic Map and Marker Information

Amphibian genomes differ greatly in DNA content and chromosome size, morphology, and number. Investigations of this diversity are needed to identify mechanisms that have shaped the evolution of vertebrate genomes. We used comparative mapping to investigate the organization of genes in the Mexican axolotl (Ambystoma mexicanum), a species that presents relatively few chromosomes (n = 14) and a gigantic genome (>20 pg/N).

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BLASTNew! We provide NCBI- BLAST (Basic Local Alignment Search Tool) to compare nucleotide or protein sequences to our EST sequence databases. BLAST can be used to infer functional and evolutionary relationships between sequences and help identify members of gene families.

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Gene Expression

The analysis of gene expression is being studied using microarray, quantitative-pcr, RNA-seq, and nanostring. These resources include:

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