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Axolotls...
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100% Holtfreter's Solution
Use at 50% strength for housing adults, 25%
for embryos.
NaCl, 3.46 g (non-iodized table salt)
KCl, 0.05 g
CaCl2, 0.1 g
NaHCO3, 0.2 g (buffer) in 1 liter of distilled water
pH adjusted to 7.4
Modified Holtfreter's
Add 0.2 g MgSO4 . 7H2O to the
recipe.
Axolotl Colony Modifications
For water in which to house axolotl adults, larvae, and embryos
developing within jelly coats, the Axolotl Colony uses tap water
which has been treated to remove chlorine, chloramines, ammonia,
etc. to make a non-buffered, non-pH-adjusted modified Holtfreter's
solution. Embryos are kept in a 20% solution (avoid concentrating
the salts through evaporation as the embryos develop), and larvae,
juveniles, and adults are kept in a 40% solution. Occasionally animals
exhibiting skin problems or other conditions will be housed temporarily
in 100% solution.
100% Steinberg's Solution
NaCl, 3.4 g
KCl, 0.05 g
Ca(NO3)2 . 4H2O, 0.08
g
MgSO4 . 7H2O, 0.205 g
Tris, 0.56 g
1 liter distilled water
HCl to pH 7.4
Reference
Asashima, M., G.M. Malacinski, and S.C. Smith. 1989. Surgical manipulation
of embryos. In J.B. Armstrong and G.M. Malacinski (eds.), Developmental
Biology of the Axolotl. Oxford University Press, New York.
pp. 255-263.
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cite The Indiana University Axolotl Colony World Wide Web Site (http://www.indiana.edu/~axolotl/)
as your source.
Copyright 1995-2000 by the Trustees
of Indiana
University . Copyright for all text is held by the author(s).
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URL: http://www.indiana.edu/~axolotl/axolotls/shortguide/solutions.html
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